Analysis Note

Low-end CV% Range: 2 - 8%|Low-end CV% Average: 4%

Application

Used to detect/quantify:IL-12

The sensitivity of Single Molecule Counting (SMC^®) gives the SMC^® Human IL-12 Immunoassay Kit unparalleled performance in the quantification of IL-12 in human EDTA plasma and serum.

Research Sub CategoryCytokines & Cytokine Receptors

Research CategoryInflammation & Immunology

Components

IL-12 Coated BeadsStandard DiluentIL-12 Detection AntibodyAssay BufferHuman IL-12 StandardSMC^® Human IL-12 Immunoassay Kit Instructions10X Wash BufferElution Buffer BBuffer D

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

General description

Interleukin-12 (IL-12) is a cytokine expressed by T-cells and natural killer cells in response to the presence of microbial agents. Increased expression of IL-12 drives a positive feedback loop, which generates more T-cells and natural killer cells, along with other immuno-regulatory cytokines such as IFN-γ, IL-10 and TNF-α. Although IL-12 is an important initiator of cell-mediated immunity, its pro-inflammatory effects can be deleterious. Over production of this cytokine has been implicated in several autoimmune disorders such as inflammatory bowel disease, psoriasis, and Crohn’;s disease. The ability to escalate and strengthen the immune response makes IL-12 an important immunostimulant therapy for infectious diseases and metastatic cancers.

The SMC^® Human IL-12 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC^® immunoassay system detects analytes using Single Molecule Counting (SMC^®) technology.

Legal Information

SMC is a registered trademark of Singulex